Tissue culture of the colorectal adenomas observing the sequence of adenoma-carcinoma continuum was tried using 2 cases from familial polyposis coli and 4 cases from adenomas with synchronous adenocarcinomas known as known as risk factors of
adenocarcinoma 9 cell lines were established through 1 to 3 subculture(s) from 11 adenomas. The growth patterns were not different in respect to size histopathological type, degree of dysplasia, and ploidy Either anchorage independent culture in
the
agar media or tumorigenicity in the nude mice was not found. 4 principal bile acids in vivo, e.g. cholic acid, chendexoycholic acid, deoxycholic acid, and lithocholic acid, of 10E-7M or 10E-8M were added into MEM-2+ media for the identification
of
proliferative effect. The colonies increased to 1.86 folds in the 10E-7M chendexoycholic acid and 1.19 folds in the 10E-8M lithocholic acid, but the colonies changed into reticular destruction due to cytolysis in the all wells of 4 bile acids
after
2
weeks. 10 cells from second subcultivated adenocarcinoma was cocultured with adenoma cells form the same host. The adenocarcinoma cells were proliferated destroying the adenoma cells completely within 48 hours.
The cultivated adenoma cells showed low biological aggressiveness and proliferation, which suggested the retarded growth and selectiveness among adenomas in the colorectal adenoma-adenocarcinoma sequence. Bile acids might affect early stages of
neoplasms. Selective predominance of adenocarcinoma comparing adenoma resulted from coculture of adenoma and adenocarcinoma cells.
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